Au pôle de protection des plantes (Réunion)
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Molecular tests

Written by Administrateur Modified on the

  • La réaction de polymérisation en chaîne (PCR)

The diagnosis of the diseases caused by viroids can only be done by serological methods. In this case techniques based on the analysis of nucleic acids in the infected plants are performed by electrophoresis on polyacrylamid gel, or on the characterization of nucleic acids by molecular hybridization.

The resort to molecular amplification, through the PCR, has allowed to extend the limits of sensitivity to diagnosis techniques based on the detection of specific sequences of nucleic acids

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